extraction du phosphate d'inositol de l'arachide en Tunisie

   
extraction du phosphate d'inositol de l'arachide en Tunisie
                                               
                                               
                                               
  • extraction du phosphate d'inositol de l'arachide en Tunisie
  • How do you extract soluble inositol polyphosphates from Arabidopsis?
  • Extraction of soluble inositol polyphosphates from Arabidopsis Add 500 μl of ice-cold extraction buffer to the 1.5 ml centrifuge tube containing the seedlings and grind using a small disposable plastic pestle. Spin down the cells at maximum speed for 10 min at 4 °C and carefully remove the supernatant to a new 1.5 ml centrifuge tube.
  • How to extract and separate inositol polyphosphates from Saccharomyces cerevisiae?
  • This protocol to extract and separate inositol polyphosphates from Saccharomyces cerevisiae is divided into three steps: labeling of yeast, extraction of soluble inositol polyphosphates and chromatographic separation. Yeast cells are incubated with tritiated inositol, which is taken up and metabolized into different phosphorylated forms.
  • Can phenol extract cyclic inositol phosphates?
  • A phenol-based extraction, which does not allow the quantitative extraction of inositol lipids, has been used to quantitatively extract the acid-labile cyclic inositol phosphates (e.g. ( 2, 3, 62 )).
  • How do you extract lipids from inositol phosphates?
  • In general, the extraction techniques usually involve an acid-based kill and separation into aqueous- and organic-soluble fractions. When inositol phosphates are extracted, a standard complementary lipid extraction protocol that is used is based on a modified acidified Bligh Dyer technique ( ( 61) and see Notes of Chapter 2 ).
  • Which inositol polyphosphate has a high turnover rate?
  • One inositol polyphosphate that fits this notion is inositol 1,2,3-trisphosphate as it labels slowly to isotopic equilibrium, but has a very high turnover rate during cell cycle progression ( 29 ). However, it should be noted that Ins (1,4,5) P 3 also exhibits this anomalous labelling.
  • How are soluble inositol polyphosphates determined?
  • Soluble inositol polyphosphates are then acid-extracted and fractionated by high-performance liquid chromatography. The radioactivity of each fraction is determined by scintillation counting. This highly sensitive and reproducible method allows the accurate detection of subtle changes in the inositol polyphosphate profile and takes less than 48 h.